RANK Rabbit Polyclonal Antibody

• Catalog Number: orb312842
• Category: Antibodies
• Description: RANK Rabbit Polyclonal Antibody
• Target: TNFRSF11A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human RANK (51-150/616aa)
• UniProt ID: Q9Y6Q6
• MW: 82-90 kDa
• Tested applications: FC, ICC
• Dilution range: ICC/IF=1:50-100, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFRSF11A; CD265; CD265 antigen; Activator of NFKB
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-RANK antibody (orb312842), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-RANK (orb312842) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63-70 kD, Observed band size: 70 kD.

Sample: U251 Cell Lysate at 30 ug, Primary: Anti-RANK (orb312842) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63-70 kD, Observed band size: 63 kD.