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Randox - Amikacin
Catalog Number: HRP9430
| Catalog Number | HRP9430 |
|---|---|
| Category | Small Molecules |
| Description | Amikacin - HRP Conjugate |
| Target | Amikacin |
| Form/Appearance | Liquid |
| Dilution range | 1/640. |
| Application notes | competitive ELISA with polyclonal antibody PAS9913/PAS9914. The conjugate is supplied as a concentrate. Only dilute the concentrate as required and use the working strength conjugate immediately after dilution. |
| Storage | This conjugate may be stored for up to 3 months at +2 - +8°C. For long term storage, aliquot and store at ≤-20°C. |
| Hazard Information | Product should be protected from light exposure. Avoid repeated freeze/thaw cycles. Avoid using azide containing buffers. Vial should be centrifuged briefly before opening to ensure all material is removed from the vial cap. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
