RAMP1 Rabbit Polyclonal Antibody

• Catalog Number: orb10480
• Category: Antibodies
• Description: RAMP1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RAMP1 (118-148/148aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: RAMP1
• UniProt ID: O60894
• RRID: AB_10753282
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: calcitonin receptor like receptor activity modifyi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Recombinant human RAMP1 protein, Primary: Anti-RAMP1 (orb10480) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: / kDa, Observed band size: 32 kDa.

Formaldehyde fixed and paraffin embedded rat kidneys labeled with Rabbit Anti-RAMP1 Polyclonal Antibody (orb10480) and followed by conjugation to a secondary antibody and counterstained with hematoxylin.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RAMP1 Polyclonal Antibody, Unconjugated (orb10480) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.