RAI14 Rabbit Polyclonal Antibody

SKU: orb312869

Description

RAI14 Rabbit Polyclonal Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RAI14 (2-100/980aa)
• Target: RAI14
• Molecular Weight: 110 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NORPEG; RAI13; 1700008J19Rik; 1700020L11Rik; Ankycorbin; mKIAA1334; RAI14_HUMAN; RAI14; Ankyrin repeat and coiled-coil structure-containing protein; Novel retinal pigment epithelial cell protein; Retinoic acid-induced protein 14; KIAA1334; RAI14_MOUSE; p125; RAI14_RAT;

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RAI14) Polyclonal Antibody, Unconjugated (orb312869) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: 293T Cell (Human) Lysate at 30 ug, Primary: Anti-RAI14 (orb312869) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110kD, Observed band size: 120kD.

Quick Database Links

Gene Symbol

RAI14

UniProt

Q9P0K7