RAGE Rabbit Polyclonal Antibody

SKU: orb155611

Description

RAGE Rabbit Polyclonal Antibody

Research Area

Alzheimer's Disease, Alzheimer's disease, Diabetes Research, Inflammation, Inflammatory Mediators, Neuroscience, Visual System

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human AGER Isoform 1, not for Isoform 2 (41-150/404aa)
• Target: Ager
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Advanced glycosylation end product specific receptor; Advanced glycosylation end product-specific receptor; AGER; EC 2.7.11.22; LE 9211 A antigen; LE-9211-A antigen; MGC22357; MOK; RAGE 1; RAGE1; MOK protein kinase; Receptor for advanced glycation endproducts; Renal tumor antigen 1; Renal tumor antigen; Renal cell carcinoma antigen(MOK protein kinase); Renal tumor antigen 1; RAGE_HUMAN.

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-RAGE (orb155611) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/43/55 kD, Observed band size: 58/50 kD.

Tissue/Cell: rat skeletal muscle, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RAGE Polyclonal Antibody, Unconjugated (orb155611) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Ager

UniProt

Q15109

References

Qiu, Yuan-Ye et al. Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy Mol Cell Endocrinol, 443, 89-105 (2017)

PubMed: 28087385