RAGE Rabbit Polyclonal Antibody

• Catalog Number: orb155611
• Category: Antibodies
• Description: RAGE Rabbit Polyclonal Antibody
• Target: Ager
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human AGER Isoform 1, not for Isoform 2 (41-150/404aa)
• UniProt ID: Q15109
• MW: 42 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Advanced glycosylation end product specific recept
• Research Area: Alzheimer's Disease, Alzheimer's disease, Diabetes
• Note: For research use only

References

Qiu, Yuan-Ye et al. Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy Mol Cell Endocrinol, 443, 89-105 (2017)

PubMed: 28087385

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-RAGE (orb155611) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/43/55 kD, Observed band size: 58/50 kD.

Tissue/Cell: rat skeletal muscle, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-RAGE Polyclonal Antibody, Unconjugated (orb155611) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.