Radixin Antibody

• Catalog Number: orb1247900
• Category: Antibodies
• Description: Radixin Antibody
• Target: RDX
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-QARDETKKTQND
• UniProt ID: P35241
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem. 1995 Oct 27;270(43):25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 2.5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RDX, radixin
• Note: For research use only
• NCBI: NP_002897.1

orb1247900 (0.03 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247900 (0.01 ug/ml) staining of NIH3T3 (A), HeLa (B), Jurkat (C), and A431 (D) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247900 (2.5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247900 (2.5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.