RAC1+RAC2 Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb103322
• Category: Antibodies
• Description: RAC1+RAC2 Rabbit Polyclonal Antibody (FITC)
• Target: RAC1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human RAC1/RAC2. (101-192/192aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1ug/test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: RAC2_HUMAN; Ras-related C3 botulinum toxin substra
• Note: For research use only

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-RAC1+RAC2 antibody (orb103322), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-RAC1+RAC2 antibody (orb103322), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.