Rabbit TNF protein

SKU: orb392582

Description

Recombinant rabbit TNF protein is produced by our E. coli using rDNA technology. The protein consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. The individual subunits have a relative molecular mass each of 17.4 Daltons.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: TNF
• Reactivity: Rabbit
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: MLRSASRALS DKPLAHVVAN PQVEGQLQWL SQRANALLAN GMKLTDNQLV VPADGLYLIY SQVLFSGQGC RSYVLLTHTV SRFAVSYPNK VNLLSAIKSP CHRETPEEAE PMAWYEPIYL GGVFQLEKGD RLSTEVNQPE YLDLAESGQV YFGIIAL

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM PB, 300mM NaCl, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Quick Database Links

Gene Symbol

TNF

UniProt

P04924

NCBI Gene ID

100009088