Rabbit TNF a Protein

• Catalog Number: orb428885
• Category: Tools
• Description: Recombinant of rabbit TNF a protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ala-Ser-Arg
• Source: Escherichia Coli
• Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.
• Storage: Stability: Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Buffer/Preservatives: TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PB, pH 7.4, 300mM NaCl.
• Alternative names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor
• Note: For research use only
• Application notes: Cytokines And Growth Factors
• Expiration Date: 12 months from date of receipt.