Rabbit polyclonal antibodies to HLA

SKU: orb2284539

Description

A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells

Images & Validation

• Tested Applications: WB
• Dilution range: Western blot: 0.1-1.0 µg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG/IgM
• Immunogen: Peptide (GDTRPRFLWQLKFECHFFNGTERVRLLER)
• Target: HLA
• Molecular Weight: 22 420
• Purification: Immunogen affinity column
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Glycine pH 7.2
• Concentration: Batch dependent within range 2.0-2.5 mg/mL
• Disclaimer: For research use only

Recombinant human HLA-DRB1 (29-221 of P01911) detected at 25 kDa by Western blotting using anti-HLA primary antibody at 1 in 2000 dilution. Lane 1: Marker; Lane 2: HLA (1ng); Lane 3: HLA (5ng); Lane 4: HLA (10ng); Lane 5: HLA (50ng); Lane 6: Blank; Lane 7: HLA (100ng); Lane 8: Blank; Lane 9: HLA (200ng); Lane 10: Blank.

Quick Database Links

Gene Symbol

HLA

UniProt

P01911

RefSeq (Protein):NP_001230894.1