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Catalog Number | orb248101 |
---|---|
Category | Antibodies |
Description | Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FACS, IF, IHC |
Isotype | Rabbit IgG |
Immunogen | This is purified naive rabbit sera and as such there was no immunogen. |
Dilution range | FACS: assay dependent,IHC: assay dependent,IF: assay dependent |
Purity | Protein A purified IgG fraction |
Conjugation | Unconjugated |
Formula | 0.5 mg/ml antibody in PBS with 0.05% sodium azide |
Hazard Information | This Rabbit isotype control antibody is available for research use only. |
Storage | Aliquot the rabbit antibody and store frozen at -20°C or colder to avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 1X PBS, pH 7.4 |
Note | For research use only |
Application notes | Generally to be used at the same concentration as the sample being controlled for. |
Expiration Date | 12 months from date of receipt. |
FACS testing of Rabbit IgG isotype control antibody on human samples. Black=cells alone, gray=cells + secondary, orange=cells + isotype control antibody + secondary.
FACS testing of Rabbit IgG isotype control antibody on mouse samples. Black=cells alone, gray=cells + secondary, orange=cells + isotype control antibody + secondary
FACS, IF, IHC | |
Rabbit | |
Polyclonal | |
FITC |
FACS, IF, IHC | |
Rabbit | |
Polyclonal | |
APC |
FACS, IF, IHC | |
Rabbit | |
Polyclonal | |
PE |
Rabbit | |
Polyclonal | |
Unconjugated |
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