Rabbit IgG Isotype Control Polyclonal Antibody FITC Conjugate

SKU: orb248103

Description

Isotype control antibodies are negative controls used to measure non-specific binding or background levels of a primary antibody in different cell types. They are not specific to any target in a cell, yet retain all non-specific characteristics of the antibodies they control.

Images & Validation

• Tested Applications: FC, IF, IHC
• Dilution range: Flow Cytometry : assay dependent| Immunohistochemistry : assay dependent| Immunofluorescence : assay dependent
• Application Notes:
  Generally to be used at the same concentration as the sample being controlled for.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This is purified naive rabbit sera and as such there was no immunogen.

Storage & Handling

• Storage: Store the Rabbit IgG isotype control antibody at 4°C. Do not freeze; protect from light.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

FACS testing of Rabbit IgG isotype control antibody FITC conjugate on human samples. Black = cells alone, green = isotype control antibody.

FACS testing of Rabbit IgG isotype control antibody FITC conjugate on mouse samples. Black = cells alone, green = isotype control antibody

References

Shuhei Nishiyama 1 2 3 4, Jin Myong Seok 5, Amy E Wright 5, Itay Lotan 5 6, Takahisa Mikami 5 6, Natalia C Drosu 5, Natasha Bobrowski-Khoury 5 6, Monique R Anderson 5 6, Philippe A Bilodeau 5 6, Patrick Schindler 7, Friedemann Paul 7, Masashi Aoki 8, Michael R Yeaman 9 10, Michael Levy Anti-aquaporin-4 immune complex stimulates complement-dependent Th17 cytokine release in neuromyelitis optica spectrum disorders Sci Rep, 14, 3146 (2024)

PubMed: 38326464

Applications

FC

Reactivity

Human