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Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Catalog Number: orb347670

DispatchUsually dispatched within 5-10 working days
$ 330.00
Catalog Numberorb347670
CategoryAntibodies
DescriptionRabbit IgG (H&L) antibody (FITC)
ClonalityPolyclonal
Species/HostGoat
IsotypeIgG
ConjugationFITC
ReactivityRabbit
Form/AppearanceLyophilized
Concentration2.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
ImmunogenRabbit IgG whole molecule
Tested applicationsIF, WB
Dilution rangeIF: 1:500 - 1:2,500
Application notesAnti-Rabbit IgG Antibody Fluorescein has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesGoat anti-Rabbit IgG Antibody fluorescein Conjugat
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NoteFor research use only
Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n orb347660). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n orb347660) 1:1000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.

Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Western Blot of Fluorescein conjugated Goat anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): none.