Rabbit IgG (H&L) antibody (ATTO 647N)

• Catalog Number: orb347698
• Category: Antibodies
• Description: Rabbit IgG (H&L) antibody (ATTO 647N)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FLISA, IF, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Rabbit IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: Rabbit IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
• Conjugation: ATTO-647N
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-Rabbit IgG Antibody ATTO647N Conjugation
• Note: For research use only
• Application notes: Anti-Rabbit IgG (H&L) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Hela (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates using Rabbit IgG (H&L) antibody (ATTO 647N)

Immunofluorescence analysis of human umbilical vein endothelial cells using Rabbit IgG (H&L) antibody (ATTO 647N)

Immunofluorescence analysis of human umbilical vein endothelial cells using Rabbit IgG (H&L) antibody (ATTO 647N)