Rabbit IgG (H&L) antibody (Alkaline Phosphatase)

• Catalog Number: orb347726
• Category: Antibodies
• Description: Rabbit IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Rabbit IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Mouse Anti-Rabbit IgG Alkaline Phosphatase Conjuga
• Note: For research use only
• Application notes: Mouse Anti-Rabbit IgG Alkaline Phosphatase Conjugate has been tested by ELISA and dot blot and is assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 is suggested for this product.
• Expiration Date: 12 months from date of receipt.