Rabbit IgG F(ab')2 Antibody

SKU: orb346311

Description

Rabbit IgG F(ab')2 Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Rabbit IgG F(ab')2 Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Key Properties

• Source: Rabbit
• Biological Origin: Rabbit
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Purity: Rabbit IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(ab’)2 and anti-Rabbit Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Immunoglobulin IgG F(ab')2 fragment, IgG Fab2

SDS PAGE gel of commercially-purified rabbit Immunoglobulin G (rIgG) and of rIgG papain digestion products Fab and Fc. Lanes C–F contain products which have been reduced with b-mercaptoethanol. Lanes H–L contain non-reduced products. Each lane contains approximately 35 mg of the following products: Lanes C and H contain papain digested products; Lanes D, I and L contain Fc fragment obtained from protein A purification; Lanes E and J contain whole rIgG; Lanes F and K contain Fab fragment obtained from protein A purification; Lanes A, B and G contain gel markers. Whole rIgG (p/n orb2652747), rFc (p/n orb346310), rFab (p/n orb2652745), rF(ab')2 (p/n orb346311).

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.