Rabbit FSH(Follicle Stimulating Hormone) ELISA Kit

• Catalog Number: orb1946468
• Category: Assays and Kits
• Description: Rabbit FSH(Follicle Stimulating Hormone) ELISA Kit
• Target: FSH
• Reactivity: Rabbit
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Follicle Stimulating Hormone(FSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Follicle Stimulating Hormone(FSH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follicle Stimulating Hormone(FSH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 3.13-200 ng/mL
• Sensitivity: 0.85 ng/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: FSH-B; FSH-beta
• Note: For research use only