You have no items in your shopping cart.
Cart summary
| Catalog Number | orb1946451 |
|---|---|
| Category | Assays and Kits |
| Description | Rabbit APOA1(Apolipoprotein A1) ELISA Kit |
| Target | APOA1 |
| Reactivity | Rabbit |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apolipoprotein A1(APOA1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein A1(APOA1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1(APOA1) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 1.5H |
| Range | 31.25-2000 ng/mL |
| Sensitivity | 10.53 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotei Read more... |
| Note | For research use only |
- Item 1 of 1
