Rabbit anti-WWP2 Antibody

• Catalog Number: orb1525732
• Category: Antibodies
• Description: WWP2 Antibody
• Target: WWP2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 200 and 250
• UniProt ID: O00308
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: Atrophin-1-interacting protein 2; WWp2-like; WW do
• Note: For research use only
• NCBI: NP_008945.2

Detection of mouse WWP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody (orb1525732) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human WWP2 by western blot. Samples: Whole cell lysate (50 µg) from SW620, MCF-7, K-562, Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody (orb1525732) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human WWP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WWP2 antibody (orb1525732) used for IP at 6 µg per reaction. WWP2 was also immunoprecipitated by a previous lot of this antibody (orb1525732) and a second antibody against a different epitope of WWP2.