Rabbit anti-USP1 Antibody

• Catalog Number: orb1527948
• Category: Antibodies
• Description: USP1 Antibody
• Target: USP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 450 and 500
• UniProt ID: O94782
• Tested applications: IP
• Dilution range: WB - Not recommended. A301-699A has not performed satisfactorily when used for WB of USP1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of USP1.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 or Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: ubiquitin specific processing protease 1; Ubiquiti
• Note: For research use only
• NCBI: NP_003359.3

Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody orb1527948 used for IP at 6 µg per reaction.

Detection of human USP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-USP1 (Cat. No. orb1527948) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB