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| Catalog Number | orb1527738 |
|---|---|
| Category | Antibodies |
| Description | TRIP12 Antibody |
| Target | TRIP12 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 75 and 125 |
| UniProt ID | Q14669 |
| Tested applications | IP, WB |
| Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | MRD49; ULF; TRIP-12; TR-interacting protein 12; Th Read more... |
| Note | For research use only |
| NCBI | NP_004229.1 |

Detection of human TRIP12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP12 antibody (orb1527738) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human TRIP12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP12 antibody (orb1527738) used for IP at 6 µg per reaction. TRIP12 was also immunoprecipitated by a previous lot of this antibody (orb1527738).
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