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Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:25 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 2313 and C-terminus |
| Target | TPR |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of human TPR by western blot.Samples: Whole cell lysate from HeLa, LNCaP, HEK293T, Hep-G2, and Jurkat cells. Antibody: Affinity purified rabbit anti-TPR antibody orb1529559 at 0.04 µg/ml.

Detection of mouse TPR by immunohistochemistry. Sample: FFPE section of mouse liver. Antibody: Affinity purified rabbit anti-TPR used at a dilution of 1:100 (2µg/ml).

Detection of human TPR by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TPR used at a dilution of 1:200 (1µg/ml).

Detection of human TPR by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-TPR antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003283.1 |
|---|
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Protocol Information
Rabbit TPR Antibody (orb1529559)
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