Rabbit TOP1 Antibody

SKU: orb1526356

Description

TOP1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 125 and 175
• Target: TOP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA topoisomerase 1; DNA topoisomerase I; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase

Detection of human TOP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TOP1 antibody orb1526356 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Quick Database Links

Gene Symbol

TOP1

UniProt

P11387

RefSeq (Protein):NP_003277.1