Rabbit anti-TOP1 Antibody

• Catalog Number: orb1526353
• Category: Antibodies
• Description: TOP1 Antibody
• Target: TOP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 715 and C-terminus
• UniProt ID: P11387
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: topoisomerase (DNA) I; type I DNA topoisomerase; D
• Note: For research use only
• NCBI: NP_003277.1

Detection of human TOP1 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa and (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody orb1526353 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody orb1526353 used for IP at 6 µg per reaction.

Detection of mouse TOP1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody orb1526353 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.