Rabbit anti-TIGIT Recombinant Monoclonal Antibody

• Catalog Number: orb1519879
• Category: Antibodies
• Description: TIGIT Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR047F
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Immunogen: Between 194 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: TIGIT
• UniProt ID: Q495A1
• NCBI: NP_776160.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: VSTM3; Washington University cell adhesion molecul
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human TIGIT by western blot. Samples: Whole cell lysate (10 µg) from HeLa, My-La CD4+, HEK293T, MJ, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519879) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human TIGIT by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519879). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human TIGIT (shaded) in MJ cells by flow cytometry. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519879) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Detection of human TIGIT (red) in immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519879). Secondary: Dylight 594 conjugated goat-anti-rabbit IgG. Counterstain: DAPI (blue).

Detection of human TIGIT (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-TIGIT recombinant monoclonal antibody (orb1519879) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).