Rabbit anti-SPF45 Antibody

• Catalog Number: orb1526517
• Category: Antibodies
• Description: SPF45 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: Between 25 and 75
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-SPF45 antibody A302-498A.; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SPF45
• UniProt ID: Q96I25
• NCBI: NP_116294.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 45 kDa-splicing factor; RNA-binding motif protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SPF45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPF45 antibody orb1526517 used for IP at 6 µg per reaction.

Detection of mouse SPF45 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. orb1526517) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human SPF45 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. orb1526517) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB