Rabbit anti-SPATA2 Antibody

• Catalog Number: orb1526523
• Category: Antibodies
• Description: SPATA2 Antibody
• Target: SPATA2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 250 and 300
• UniProt ID: Q9UM82
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: spermatogenesis associated PD1; tamo; spermatogene
• Note: For research use only
• NCBI: NP_006029.1

Detection of human SPATA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SPATA2 antibody orb1526523 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. SPATA2 was also immunoprecipitated by rabbit anti-SPATA2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human SPATA2 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPATA2 (Cat. No. orb1526523) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB