Rabbit anti-SF3A3 Antibody

SKU: orb1526498

Description

SF3A3 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 451 and C-terminus
• Target: SF3A3
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

spliceosome associated protein 61; splicing factor 3a, subunit 3, 60kDa; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD); Spliceosome-associated protein 61; SF3a60; SAP61; SAP 61; PRPF9; PRP9; splicing factor 3A subunit 3

Detection of human and mouse SF3A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SF3A3 antibody orb1526498 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. SF3A3 was also immunoprecipitated by rabbit anti-SF3A3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human SF3A3 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SF3A3 (Cat. No. orb1526498) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SF3A3

UniProt

Q12874

RefSeq (Protein):NP_006793.1