Rabbit anti-SCAP Antibody

• Catalog Number: orb1524611
• Category: Antibodies
• Description: SCAP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 450 and 500
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SCAP
• UniProt ID: Q12770
• NCBI: NP_036367.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SCAP; SREBP cleavage-activating protein; sterol re
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SCAP by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Hep-G2, HeLa, Jurkat, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCAP antibody (orb1524611) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SCAP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SCAP antibody (orb1524611) used for IP at 6 µg per reaction.