Rabbit SAM68 Antibody

SKU: orb1527200

Description

SAM68 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: SAM68
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAP-associated tyrosine phosphoprotein p62; GAP-associated tyrosine phosphoprotein p62 (Sam68); KH domain containing, RNA binding, signal transduction associated 1; KH domain-containing, RNA-binding, signal transduction-associated protein 1; p21 Ras GTPase-activating protein-associated p62; p62; p68; Sam68; src-associated in mitosis 68 kDa protein

Detection of human SAM68 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAM68 antibody orb1527200 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of human SAM68 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SAM68 (Cat. No. orb1527200) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SAM68

UniProt

Q07666

RefSeq (Protein):NP_006550.1