Rabbit anti-RSK3 Antibody

• Catalog Number: orb1523355
• Category: Antibodies
• Description: RSK3 Antibody
• Target: RSK3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 1 and 50
• UniProt ID: Q15349
• Tested applications: IHC, IP
• Dilution range: WB - Not recommended. A304-243A has not performed satisfactorily when used for WB of RSK3 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RSK3.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: pp90RSK3; S6K-alpha2; S6K-alpha; RSK-3; RSK3; RSK;
• Note: For research use only
• NCBI: NP_066958.2

Detection of human RSK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RSK3 antibody orb1523355 used for IP at 6 µg/mg lysate.

Detection of human RSK3 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RSK3 (Cat. No. orb1523355) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).