Rabbit anti-RPB3 Antibody

• Catalog Number: orb1524210
• Category: Antibodies
• Description: RPB3 Antibody
• Target: RPB3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 225 and C-terminus
• UniProt ID: P19387
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: polymerase (RNA) II subunit C; RNA polymerase II s
• Note: For research use only
• NCBI: NP_116558.1

Detection of human RPB3 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPB3 antibody (orb1524210) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of mouse RPB3 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPB3 antibody (orb1524210) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPB3 antibody (orb1524210) used for IP at 6 µg per reaction. RPB3 was also immunoprecipitated by a previous lot of this antibody (orb1524210) and a second antibody against a different epitope of RPB3.

Detection of human RPB3 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPB3 (Cat. No. orb1524210) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB