Rabbit anti-ROC1 Antibody

SKU: orb1524791

Description

ROC1 Antibody

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 1 and 50
• Target: ROC1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

RING finger protein 75; ZYP protein; ROC1; RNF75; RING-box protein 1; regulator of cullins 1; Rbx1; Protein ZYP; E3 ubiquitin-protein transferase RBX1; E3 ubiquitin-protein ligase RBX1; BA554C12.1; ring-box 1, E3 ubiquitin protein ligase

Detection of human and mouse ROC1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-ROC1 antibody orb1524791 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ROC1 by immunohistochemistry.Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-ROC1 (Cat. No. orb1524791) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human ROC1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ROC1 (Cat. No. orb1524791) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ROC1

UniProt

P62877

RefSeq (Protein):NP_055063.1