You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Rabbit anti-RNF25 Antibody
Catalog Number: orb1524077
| Catalog Number | orb1524077 |
|---|---|
| Category | Antibodies |
| Description | RNF25 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 409 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | RNF25 |
| UniProt ID | Q96BH1 |
| NCBI | NP_071898.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | ring finger protein AO7; RING-type E3 ubiquitin tr Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RNF25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for IP at 6 µg/mg lysate.
- Item 1 of 2
