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Rabbit anti-RNF214 Antibody
Catalog Number: orb1522502
| Catalog Number | orb1522502 |
|---|---|
| Category | Antibodies |
| Description | RNF214 Antibody |
| Target | RNF214 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 1000 µg/ml |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Immunogen | Between 1 and 50 |
| UniProt ID | Q8ND24 |
| Tested applications | IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | RING finger protein 214 |
| Note | For research use only |
| NCBI | NP_001070707.1 |
Detection of human RNF214 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF214 antibody orb1522502 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RNF214 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF214 antibody orb1522502 used for IP at 6 µg per reaction.
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