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Catalog Number | orb1523617 |
---|---|
Category | Antibodies |
Description | RNF169 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP |
Reactivity | Human |
Immunogen | Between 200 and 250 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. A304-097A has not performed satisfactorily when used for WB of RNF169 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNF169.; IP - 2 - 5 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RNF169 |
UniProt ID | Q8NCN4 |
NCBI | NP_001092108.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | E3 ubiquitin-protein ligase RNF169; RING finger pr Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human RNF169 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF169 antibody orb1523617 used for IP at 3 µg per reaction.