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Rabbit anti-RecQ1 Antibody
Catalog Number: orb1530190
| Catalog Number | orb1530190 |
|---|---|
| Category | Antibodies |
| Description | RecQ1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 1 and 50 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:1,000 - 1:10,000; IP - Not recommended. A300-450A is recommended for IP. In initial tests, A300-447A was less efficient.; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | RecQ1 |
| UniProt ID | P46063 |
| NCBI | NP_116559.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | DNA helicase, RecQ-like type 1; DNA-dependent ATPa Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human RecQ1 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti-RecQ1 used at a dilution of 1:200 (1µg/ml).
Detection of human and mouse RecQ1 by WB and human ReqQ1 by IP.Samples: A) human (HeLa) and mouse (NIH 3T3) cells. B) HeLa cells. Antibodies: Rabbit anti-RecQ1 antibody used at 0.1 µg/ml (A), 1 µg/ml (B) for WB and at 3 µg/mg lysate for IP (B).
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