Rabbit anti-PTEN Recombinant Monoclonal Antibody

• Catalog Number: orb1784570
• Category: Antibodies
• Description: Rabbit anti-PTEN Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR270L
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Between 353 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 100 µg/ml
• Dilution range: WB: 1:1000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PTEN
• UniProt ID: P60484
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: mutated in multiple advanced cancers 1; mitochondr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse and rat PTEN by western blot. Samples: Whole cell lysate (50 µg) from 70Z/3, NIH 3T3, Ba/F3, EL4, and C6 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784570) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human PTEN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, OVCAR-3, SR, MCF-7, and NCI-H460 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784570) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.