Rabbit anti-PSMD12 Antibody

• Catalog Number: orb1524105
• Category: Antibodies
• Description: PSMD12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - Not recommended
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PSMD12
• UniProt ID: O00232
• NCBI: NP_002807.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: 26S proteasome regulatory subunit RPN5; STISS; Rpn
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PSMD12 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD12 antibody orb1524105 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PSMD12 was more efficiently immunoprecipitated by rabbit anti-PSMD12 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).