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Rabbit anti-PolA2 Antibody
Catalog Number: orb1526550
| Catalog Number | orb1526550 |
|---|---|
| Category | Antibodies |
| Description | PolA2 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IP, WB |
| Reactivity | Human |
| Immunogen | Between 548 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:1,000 - 1:5,000; IP - 5 - 15 µg/mg lysate |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | PolA2 |
| UniProt ID | Q14181 |
| NCBI | NP_002680.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | DNA polymerase alpha 70 kDa subunit; polymerase (D Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human PolA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PolA2 antibody orb1526550 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. PolA2 was also immunoprecipitated by rabbit anti-PolA2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 10 seconds (B).
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