Rabbit anti-PLK1 Antibody

• Catalog Number: orb1530466
• Category: Antibodies
• Description: PLK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: Between 325 and 375
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 4 µg/mg lysate; ICC-IF - 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PLK1
• UniProt ID: P53350
• NCBI: NP_005021.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PLK; serine/threonine-protein kinase 13; STPK13; s
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PLK1 by western blot.Samples: Whole cell lysate from HeLa and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-PLK1 antibody orb1530466 at 0.1 µg/ml.

Detection of human PLK1 by WB and IP.Samples: HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody orb1530466 used at the indicated concentrations for WB.

Detection of human PLK1 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-PLK1 used at a dilution of 1:1, 000. Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.