Rabbit PHF8 Antibody

SKU: orb1527819

Description

PHF8 Antibody

Research Area

Cancer Biology, Neuroscience

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 675 and 725
• Target: PHF8
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

histone lysine demethylase PHF8; JHDM1F; jumonji C domain-containing histone demethylase 1F; KDM7B; MRXSSD; PHD finger protein 8; ZNF422

Detection of human and mouse PHF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat, HeLa, HEK293T, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF8 antibody orb1527819 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PHF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF8 antibody orb1527819 used for IP at 6 µg per reaction.

Detection of human PHF8 by immunohistochemistry. Samples: FFPE serial sections of human small cell lung cancer. Antibody: Affinity purified rabbit anti-PHF8 (Cat. No. orb1527819) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Detection of mouse PHF8 by immunohistochemistry.Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-PHF8 (Cat. No. orb1527819) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PHF8

UniProt

Q9UPP1

RefSeq:CAI41576.1