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Catalog Number | orb1528567 |
---|---|
Category | Antibodies |
Description | Pericentrin/Kendrin Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IP, WB |
Reactivity | Human |
Immunogen | Between 175 and 225 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; ICC-IF - 1:1,000 - 1:5,000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Pericentrin/Kendrin |
UniProt ID | O95613 |
NCBI | NP_006022.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | pericentrin; PCTN2; SCKL4; pericentrin-B; pericent Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Pericentrin-Kendrin by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Hep-G2, and K-562 cells. Antibody: Affinity purified rabbit anti-Pericentrin-Kendrin antibody at 0.04 µg/ml.
Detection of human Pericentrin-Kendrin by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-Pericentrin-Kendrin antibody.
ICC: Sample: HeLa cells. Antibody: Rabbit anti-Pericentrin/Kendrin at 1:1, 000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated. Counterstain. DAPI (blue) and Phalloidin (green).
ICC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |