Rabbit anti-PARN Antibody

• Catalog Number: orb1524594
• Category: Antibodies
• Description: PARN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 589 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PARN
• UniProt ID: O95453
• NCBI: NP_002573.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: deadenylation nuclease; polyadenylate-specific rib
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PARN by western blot. Samples: Whole cell lysate (10 µg) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (orb1524594) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse PARN by western blot. Samples: Whole cell lysate (10 µg) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (orb1524594) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PARN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARN antibody (orb1524594) used for IP at 6 µg per reaction. PARN was also immunoprecipitated by a previous lot of this antibody (orb1524594) and a second antibody against a different epitope of PARN.

Detection of human PARN by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PARN (orb1524594) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).