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Rabbit anti-NRIP Antibody
Catalog Number: orb1526631
| Catalog Number | orb1526631 |
|---|---|
| Category | Antibodies |
| Description | NRIP Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP |
| Reactivity | Human |
| Immunogen | Between 300 and 350 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - Not recommended. Use rabbit anti-NRIP antibody A302-435A.; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:800. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | NRIP |
| UniProt ID | Q58WW2 |
| NCBI | NP_001017977.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | IQ motif and WD repeat-containing protein 1; nucle Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human NRIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NRIP antibody (orb1526631) used for IP at 6 µg per reaction. NRIP was also immunoprecipitated by a previous lot of this antibody (orb1526631) and a second antibody against a different epitope of NRIP.
Detection of human NRIP by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NRIP (orb1526631) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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