Rabbit anti-NRF Antibody

• Catalog Number: orb1523747
• Category: Antibodies
• Description: NRF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 50 and 100
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NRF
• UniProt ID: O15226
• NCBI: NP_001166959.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ITBA4; NF-kappa-B-repressing factor; NFkB-repressi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NRF by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-NRF antibody orb1523747 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NRF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NRF antibody orb1523747 used for IP at 6 µg/mg lysate.

Detection of human NRF by immunohistochemistry.Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-NRF (Cat. No. orb1523747) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB