Rabbit anti-Notch2 Antibody

• Catalog Number: orb1527247
• Category: Antibodies
• Description: Notch2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 2421 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Notch2
• UniProt ID: Q04721
• NCBI: NP_077719.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: AGS2; Notch homolog 2; Notch 2; neurogenic locus n
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Notch2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Notch2 antibody orb1527247 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human Notch2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Notch2 (Cat. No. orb1527247) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human Notch2 (shaded) in U931 cells by flow cytometry.Antibody: Rabbit anti-Notch2 polyclonal antibody (orb1527247) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.