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Rabbit anti-NOL12 Antibody
SKU: orb1526096
Description
Images & Validation
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| Tested Applications | IHC, IP |
|---|---|
| Dilution range | WB - Not recommended. Use rabbit anti-NOL12 antibody A302-733A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Between 75 and 125 |
| Target | NOL12 |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−dJ37E16.7; Nop25; nucleolar protein 12
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Detection of human NOL12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody orb1526096 used for IP at 3 µg/mg lysate.
Detection of human NOL12 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (Cat. No. orb1526096) used at a dilution of 1: 1, 000 (1µg/ml) Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_077289.1 |
|---|
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Protocol Information
IHC
Immunohistochemistry
IP
Immunoprecipitation
Rabbit anti-NOL12 Antibody (orb1526096)
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