Rabbit MED16 Antibody

SKU: orb1524413

Description

MED16 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 550 and 600
• Target: MED16
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DRIP92; Mediator complex subunit 16; mediator of RNA polymerase II transcription subunit 16; THRAP5; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein complex 95 kDa component; thyroid hormone receptor-associated protein, 95-kD subunit; TRAP95; vitamin D3 receptor-interacting protein complex 92 kDa component

Detection of human MED16 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MED16 antibody orb1524413 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. MED16 was also immunoprecipitated by rabbit anti-MED16 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Quick Database Links

Gene Symbol

MED16

UniProt

Q9Y2X0

RefSeq (Protein):NP_005472.2