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Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2-10 µl/mg lysate |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 335 and C-terminus |
| Target | LSM14B |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Detection of human LSM14B by western blot. Samples: Whole cell lysate (25 µg) from Jurkat, MCF-7, U2OS, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LSM14B antibody (orb1520317) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (orb1520317) used for IP at 6 µg per reaction.

Detection of human LSM14B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LSM14B antibody (orb1520317) used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_653304.2 |
|---|
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Protocol Information
Rabbit LSM14B Antibody (orb1520317)
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